The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches

Macromolecular complexes of proteins and RNAs are essential building blocks cells. These stable supramolecular particles can be viewed as minimal biochemical units whose structural organization, i.e., the way RNA protein interact with each other, is directly linked to their biological function. Whether those dynamic regulatory ribonucleoproteins (RNPs) or integrated molecular machines involved in gene expression, comprehensive knowledge these critical our understanding key mechanisms cell physiology phenomena. Such goal diverse complexomic approaches particular recently developed gradient profiling by sequencing (Grad-seq). By separating cellular on a density quantifying distributions genome-wide mass spectrometry deep sequencing, Grad-seq charts global landscapes native macromolecular assemblies. In this review, we propose function-based ontology RNPs discuss how related transformed perspective bacterial eukaryotic guiding discovery new RNA-binding unusual classes noncoding RNAs. We highlight some methodological aspects developments that permit further boost power technique look for exciting biology understudied challenging models.